Fluorescein Clearance Kinetics in Blood and Bile Indicates Hepatic Ischemia-Reperfusion Injury in Rats
American Physiological Society
American Journal of Physiology: Gastrointestinal and Liver Physiology
Original Item ID
Quantitative measurement of the degree of hepatic ischemia-reperfusion injury (IRI) is crucial for developing therapeutic strategies for its treatment. We hypothesized that clearance of fluorescent dye through bile metabolism may reflect the degree of hepatic IRI. In this study, we investigated sodium fluorescein clearance kinetics in blood and bile for quantifying the degree of hepatic IRI. Warm ischemia times (WITs) of 0, 30, or 60 min followed by 1 h or 4 h of reperfusion, were applied to the median and lateral lobes of the liver in Sprague-Dawley rats. Subsequently, 2 mg/kg of sodium fluorescein was injected intravenously, and blood and bile samples were collected over 60 min to measure fluorescence intensities. The bile-to-plasma fluorescence ratios demonstrated an inverse correlation with WIT and were distinctly lower in the 60-min WIT group than in the control or 30-min WIT groups. Bile-to-plasma fluorescence ratios displayed superior discriminability for short versus long WITs when measured 1 h after reperfusion versus 4 h. We conclude that the bile-to-blood ratio of fluorescence after sodium fluorescein injection has the potential to enable the quantification of hepatic IRI severity.
Kim, Joohyun; Yang, Yongquiang; Hong, Seung-Keun; Zielonka, Jacek; Dash, Ranjan K.; Audi, Said H.; Kumar, Suresh N.; Joshi, Amit; Zimmerman, Michael W.; and Hong, Johnny C., "Fluorescein Clearance Kinetics in Blood and Bile Indicates Hepatic Ischemia-Reperfusion Injury in Rats" (2022). Biomedical Engineering Faculty Research and Publications. 661.
Available for download on Friday, September 01, 2023
Accepted version. American Journal of Physiology: Gastrointestinal and Liver Physiology, Vol. 323, No. 2 (August 2022): G126-G133. DOI. © 2022 American Physiological Society. Used with permission.