An Interpretation of 14C-urea and 14C-primidone Extraction in Isolated Rabbit Lungs
Format of Original
Annals of Biomedical Engineering
Original Item ID
We measured the venous concentration versus time curves of14C-urea and14C-primidone after rapid bolus injections of a vascular reference indicator, fluorescein isothiocyanate dextran, and one of the two14C-labeled indicators in isolated rabbit lungs perfused with Krebs-Ringer bicarbonate solution containing 4.5% bovine serum albumin at flow rates (F) of 6.67, 3.33, 1.67, and 0.83 ml/sec and with nearly constant microvascular pressure and total lung vascular volume. When we calculated the permeability-surface area product, PS, from the14C-urea and14C-primidone outflow curves using the Crone model, the estimates of the PS product were directly proportional to F. However, the fractional change in the PS with flow was different for the two indicators. We also estimated the PS from the same14C-urea and14C-primidone data using an alternative model that includes perfusion heterogeneity, estimated in a previous study, and flow-limited and barrier-limited extravascular volumes accessible to both urea and primidone. This model was able to fit the outflow curves of either14C-urea or14C-primidone at all four flows studied with one flow-independent PS for each indicator. The ability of the new model to explain the14C-urea and14C-primidone data with no flow-dependent change in PS suggests that a change in PS with F estimated using other models such as the Crone model is not sufficient evidence for capillary surface area recruitment.
Audi, Said H.; Dawson, C. A.; Linehan, J. H.; Krenz, Gary S.; Ahlf, S. B.; and Roerig, David L., "An Interpretation of 14C-urea and 14C-primidone Extraction in Isolated Rabbit Lungs" (1996). Biomedical Engineering Faculty Research and Publications. 7.