Evidence for Intramolecular Cross-Linked Aα·γ Chain Heterodimers in Plasma Fibrinogen

Authors

Kevin R. Siebenlist, Marquette UniversityFollow
Michael W. Mosesson, Blood Center of WisconsinFollow

Document Type

Article

Publication Date

5-1996

Publisher

American Chemical Society

Source Publication

Biochemistry

Source ISSN

0006-2960

Original Item ID

DOI: 10.1021/bi952264h

Abstract

A peptide band of ∼105 kDa migrating near the γ dimer position of disulfide bond reduced human plasma fibrinogen prepared from fresh single donor or outdated plasma was identified by SDS−PAGE. The band, amounting to ∼2% of the total Aα/γ chain population, was thrombin and plasmin sensitive and reacted with antibodies to Aα or γ chains but not with antibodies to Bβ chains, plasminogen, or factor XIII. Amino acid sequencing revealed a double sequence corresponding to that of Aα and γ chains, indicating that the band consists of covalently cross-linked Aα·γ chain heterodimers. Aα·γ heterodimers were identified as a component of monomeric fibrinogen by two-dimensional SDS−PAGE and by SDS−PAGE analysis of the monomer fraction isolated by gel sieving chromatography, thus indicating that Aα·γ heterodimers arise by intramolecular Aα/γ chain cross-linking.

Comments

Accepted version. Biochemistry, Vol. 53, No. 18 (May 1996): 5817-5821. DOI. © 1996 American Chemical Society. Used with permission.

Recommended Citation

Siebenlist, Kevin R. and Mosesson, Michael W., "Evidence for Intramolecular Cross-Linked Aα·γ Chain Heterodimers in Plasma Fibrinogen" (1996). Biomedical Sciences Faculty Research and Publications. 247.
https://epublications.marquette.edu/biomedsci_fac/247