Vasopressin Resets the Central Circadian Clock in a Manner Influenced by Sex and Vasoactive Intestinal Polypeptide Signaling

Authors

Kayla E. Rohr, Marquette University
Thomas Inda, Marquette University
Jennifer A. Evans, Marquette UniversityFollow

Document Type

Article

Publication Date

8-2022

Publisher

Karger

Source Publication

Neuroendocrinology

Source ISSN

0028-3835

Original Item ID

DOI: 10.1159/000521286

Abstract

Background/Aims: Circadian rhythms in behavior and physiology are programmed by the suprachiasmatic nucleus (SCN) of the hypothalamus. A subset of SCN neurons produce the neuropeptide arginine vasopressin (AVP), but it remains unclear whether AVP signaling influences the SCN clock directly.

Methods: Here, we test that AVP signaling acting through V1A and V1B receptors influences molecular rhythms in SCN neurons. V1 receptor agonists were applied ex vivo to PERIOD2::LUCIFERASE SCN slices, allowing for real-time monitoring of changes in molecular clock function.

Results: V1A/B agonists reset the phase of the SCN molecular clock in a time-dependent manner, with larger magnitude responses by the female SCN. Further, we found evidence that both G_αq and G_αs signaling pathways interact with V1A/B-induced SCN resetting, and that this response requires vasoactive intestinal polypeptide (VIP) signaling.

Conclusions: Collectively, this work indicates that AVP signaling resets SCN molecular rhythms in conjunction with VIP signaling and in a manner influenced by sex. This highlights the utility of studying clock function in both sexes and suggests that signal integration in central clock circuits regulates emergent properties important for the control of daily rhythms in behavior and physiology.

Comments

Accepted version. Neuroendocrinology, Vol. 112, No. 9 (August 2022): 904-916. DOI. © 2022 Karger. Used with permission.

Recommended Citation

Rohr, Kayla E.; Inda, Thomas; and Evans, Jennifer A., "Vasopressin Resets the Central Circadian Clock in a Manner Influenced by Sex and Vasoactive Intestinal Polypeptide Signaling" (2022). Biomedical Sciences Faculty Research and Publications. 253.
https://epublications.marquette.edu/biomedsci_fac/253