Interactions of Water-soluble Metalloporphyrins with Nucleic Acids Studied by Resonance Raman Spectroscopy

Authors

Jinghua H. Schneider, Marquette University
Junichi Odo, Marquette University
Kazuo Nakamoto, Marquette University

Document Type

Article

Publication Date

11-1988

Source Publication

Nucleic Acids Research

Source ISSN

0305-1048

Abstract

The resonance Raman spectra of water-soluble porphyrins, M(TMpy-P4) (M = Cu(II), Ni(II) and Co(III)) and their mixtures with poly(dG-dC)₂, poly(dA-dT)₂ and calf thymus and salmon DNAs were measured using a divided rotating cell to determine the magnitudes of frequency shift and intensity variation resulting from M(TMpy-P4)-nucleic acid interactions. Bands II(Cβ-H bending, ˜ 1100 cm-1) and VIII(Cβ-Cβ stretch, ˜ 1570 cm-1) show a large and small upward shift, respectively, when Cu(TMpy-P4) and Ni(TMpy-P4) are intercalated at the G-C sites. In contrast, these bands show a small upward and downward shift, respectively, when Co(TMpy-P4) is groove-bound at the A-T sites of nucleic acids. Both Bands V (˜ 1260 cm-1) and IX (˜ 1646 cm-1) which originate in the N-methylpyridyl group always show small downward shifts due to coulombic interaction between the N-CH₃+ group of TMpy-P4 and the PO₂ group of the nucleic acid.

Comments

Published version. Nucleic Acids Research, Vol. 16, No. 21 (November 1988): 10323-10338. DOI. © 1988 Oxford University Press. Used with permission.

Recommended Citation

Schneider, Jinghua H.; Odo, Junichi; and Nakamoto, Kazuo, "Interactions of Water-soluble Metalloporphyrins with Nucleic Acids Studied by Resonance Raman Spectroscopy" (1988). Chemistry Faculty Research and Publications. 381.
https://epublications.marquette.edu/chem_fac/381