Document Type
Article
Language
eng
Publication Date
10-2020
Publisher
Elsevier
Source Publication
Journal of Oral Biology and Craniofacial Research
Source ISSN
2212-4268
Abstract
Purpose
Over the past decades, a variety of biomaterials have been investigated in terms of their suitability for oral mucosa tissue engineering. The aim of this study was to compare collagen and GelMA hydrogels as connective tissue scaffolds for fibroblasts and as substrates for seeding and culture of oral epithelial keratinocyte cells.
Methods
Human primary oral fibroblast and keratinocyte cells were isolated from gingival biopsies. The mixture of fibroblasts with GelMA or collagen gel were aliquoted within six-well tissue culture plate inserts and cross-linked using visible light or reconstitution buffer/heat, respectively. The viability of fibroblasts in the hydrogels was investigated after one and three days of cultivation using the PrestoBlue assay. Following the addition and culture of oral keratinocytes onto the connective tissue constructs, the tissue-engineered oral mucosa was assessed histologically.
Results
The tissue viability assay shows that collagen hydrogels encapsulating fibroblasts displayed significantly higher cell viability than cell-laden GelMA constructs after 24 and 72 h (p < 0.05). A stratified and differentiated epithelium has formed on the surface of cell-laden collagen hydrogel but not on the surface of the GelMA-based substrate.
Conclusion
Collagen-based scaffold offers superior biological properties compared to GelMA hydrogel in terms of oral fibroblast growth, as well as epithelial cell adhesion and differentiation. Therefore, collagen-based hydrogels remain the preferred choice for oral mucosa tissue engineering.
Recommended Citation
Tabatabaei, Fahimeh; Moharamzadeh, Keyvan; and Tayebi, Lobat, "Fibroblast Encapsulation in Gelatin Methacryloyl (GelMA) Versus Collagen Hydrogel as Substrates for Oral Mucosa Tissue Engineering" (2020). School of Dentistry Faculty Research and Publications. 406.
https://epublications.marquette.edu/dentistry_fac/406
Comments
Accepted version. Journal of Oral Biology and Craniofacial Research, Vol. 10, No. 4 (October-December 2020): 573-577. DOI. © 2020 Elsevier. Used with permission.