Document Type
Article
Language
eng
Publication Date
11-2020
Publisher
Korean Academy of Conservative Dentistry
Source Publication
Restorative Dentistry & Endodontics
Source ISSN
2234-7658
Abstract
Abstract
Objective
The aim of this study was to introduce a gelatin/bovine serum albumin (BSA) tissue standard, which provides dissolution properties identical to those of biological tissues. Further, the study evaluated whether the utilization of endodontic activating devices led to enhanced phantom dissolution rates.
Materials and Methods
Bovine pulp tissue was obtained to determine a benchmark of tissue dissolution. The surface area and mass of samples were held constant while the ratio of gelatin and BSA were varied, ranging from 7.5% to 10% gelatin and 5% BSA. Each sample was placed in an individual test tube that was filled with an appropriate sodium hypochlorite solution for 1, 3, and 5 minutes, and then removed from the solution, blotted dry, and weighed again. The remaining tissue was calculated as the percent of initial tissue to determine the tissue dissolution rate. A radiopaque agent (sodium diatrizoate) and a fluorescent dye (methylene blue) were added to the phantom to allow easy quantification of phantom dissolution in a canal block model when activated using ultrasonic (EndoUltra) or sonic (EndoActivator) energy.
Results
The 9% gelatin + 5% BSA phantom showed statistically equivalent dissolution to bovine pulp tissue at all time intervals. Furthermore, the EndoUltra yielded significantly more phantom dissolution in the canal block than the EndoActivator or syringe irrigation.
Conclusions
Our phantom is comparable to biological tissue in terms of tissue dissolution and could be utilized for in vitro tests due to its injectability and detectability.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Recommended Citation
Khoshroo, Kimia; Shah, Brinda; Johnson, Alexander; Baeten, John; Barry, Katherine; Tahiri, Mohammadreza; Ibrahim, Mohamed; and Tayebi, Lobat, "A New Phantom to Evaluate the Tissue Dissolution Ability of Endodontic Irrigants and Activating Devices" (2020). School of Dentistry Faculty Research and Publications. 411.
https://epublications.marquette.edu/dentistry_fac/411
Comments
Published version. Restorative Dentistry & Endodontics, Vol. 45, No. 4 (November 2020): e45. DOI. © 2020 Korean Academy of Conservative Dentistry. Used with permission.