The Adverse Effects of Radiotherapy on the Structure of Dental Hard Tissues and Longevity of Dental Restoration
Taylor & Francis
International Journal of Radiation Biology
The main goal of this study was to evaluate the impact of different ionizing radiation doses on the mineral (carbonate/phosphate ratio, crystallinity index [CI]) and organic (amide III/phosphate, amide I sub-band ratios) structures, as well as the microhardness, of enamel and dentin, along with their influence on the bonding strength stability of the etch-and-rinse (ER) and self-etch (SE) dental adhesive strategies.
Materials and methods:
Enamel and dentin human tissue specimens were irradiated (with 0, 20, 40, and 70 Gy radiation doses, respectively) and sectioned to perform an attenuated total reflection-Fourier transform IR spectroscopy assay (ATR-FTIR) and the Vickers microhardness (VHN) test to conduct a biochemical and biomechanical evaluation of the tissues. Regarding the adhesive properties, restored enamel and dentin specimens exposed to the same radiation doses were submitted to microshear bond strength (μSBS) tests for enamel in immediate time (IM) and to microtensile bond strength (μTBS) tests after for IM and 12-month (12 M) period of time, Mann–Whitney U tests were implemented, using the ATR-FTIR data for significant differences (α < 0.05), and three- and two-way analyses of variance, along with post-testing, were performed on the μTBS and μSBS data (MPa), respectively (Tukey post hoc test at α = 0.05).
The ATR-FTIR results showed a significant decrease (p
It is possible to conclude that structural alterations of enamel and dentin are generated by all radiation doses, decreasing the microhardness of dental hard tissues and influencing bond strength over time, starting at 40 Gy radiation dose. The etch-and-rinse strategy demonstrates better adhesive performance but generates cohesive fractures in the enamel.
Munoz, Miguel Angel; Garin-Correa, Carolina; Gonzalez-Arriagada, Wilfredo; Davila, Ximena Quintela; Haberle, Patricio; Bedran-Russo, Ana K.; and Luque-Martinez, Issis, "The Adverse Effects of Radiotherapy on the Structure of Dental Hard Tissues and Longevity of Dental Restoration" (2020). School of Dentistry Faculty Research and Publications. 486.
ADA Accessible Version
Accepted version. International Journal of Radiation Biology, Vol. 96, No. 7 (2020, July): 910-918. DOI. © 2020 Taylor & Francis. Used with permission.