Authors

María Muñoz-Amatriaín, University of California - Riverside
Stefano Lonardi, University of California - Riverside
MingCheng Luo, University of California - Davis
Kavitha Madishetty, University of California - Riverside
Jan T. Svensson, University of California - Riverside
Matthew J. Moscou, University of California - Riverside
Steve Wanamaker, University of California - Riverside
Tao Jiang, University of California - Riverside
Andris Kleinhofs, Washington State University
Gary J. Muehlbauer, University of Minnesota - St. Paul
Roger P. Wise, Iowa State University
Nils Stein, Leibniz Institute of Plant Genetics and Crop Plant Research
Shane Ma, University of California - Riverside
Edmundo Rodriguez, University of California - Riverside
Dave Kudrna, University of Arizona
Prasanna R. Bhat, University of California - Riverside
Shiaoman Chao, USDA-ARS Biosciences Research Lab
Pascal Condamine, University of California - Riverside
Shane Heinen, University of Minnesota - St. Paul
Josh Resnik, University of California - Riverside
Rod Wing, University of Arizona
Heather N. Witt, University of California - Davis
Matthew Alpert, University of California - Riverside
Marco Beccuti, University of California - Riverside
Serdar Bozdag, Marquette UniversityFollow
Francesca Cordero, University of California - Riverside
Hamid Mirebrahim, University of California - Riverside
Rachid Ounit, University of California - Riverside
Yonghui Wu, University of California - Riverside
Frank You, USDA-ARS
Jie Zheng, University of California - Riverside
Hana Simková, Institute of Experimental Botany
Jaroslav Dolezel, Institute of Experimental Botany
Jane Grimwood, DOE Joint Genome Institute
Jeremy Schmutz, DOE Joint Genome Institute
Denisa Duma, University of California - Riverside
Lothar Altschmied, Leibniz Institute of Plant Genetics and Crop Plant Research
Tom Blake, Montana State University - Bozeman
Phil Bregitzer, USDA-ARS
Laurel Cooper, Oregon State University
Muharrem Dilbirligi, Washington State University
Anders Falk, Swedish University of Agricultural Sciences
Leila Feiz, Montana State University - Bozeman
Andreas Graner, Leibniz Institute of Plant Genetics and Crop Plant Research
Perry Gustafson, University of Missouri
Patrick M. Hayes, Oregon State University
Peggy Lemaux, University of California - Berkeley
Jafar Mammadov, Virginia Tech
Timothy J. Close, University of California - Riverside

Document Type

Article

Language

eng

Format of Original

12 p.; 28 cm

Publication Date

10-2015

Publisher

Wiley

Source Publication

Plant Journal

Source ISSN

0960-7412

Original Item ID

Shelves: QK 728 .P53 2015 v. 84, Raynor Memorial Periodicals; doi: 10.1111/tpj.12959

Abstract

Barley (Hordeum vulgare L.) possesses a large and highly repetitive genome of 5.1 Gb that has hindered the development of a complete sequence. In 2012, the International Barley Sequencing Consortium released a resource integrating whole-genome shotgun sequences with a physical and genetic framework. However, because only 6278 bacterial artificial chromosome (BACs) in the physical map were sequenced, fine structure was limited. To gain access to the gene-containing portion of the barley genome at high resolution, we identified and sequenced 15 622 BACs representing the minimal tiling path of 72 052 physical-mapped gene-bearing BACs. This generated ~1.7 Gb of genomic sequence containing an estimated 2/3 of all Morex barley genes. Exploration of these sequenced BACs revealed that although distal ends of chromosomes contain most of the gene-enriched BACs and are characterized by high recombination rates, there are also gene-dense regions with suppressed recombination. We made use of published map-anchored sequence data from Aegilops tauschii to develop a synteny viewer between barley and the ancestor of the wheat D-genome. Except for some notable inversions, there is a high level of collinearity between the two species. The software HarvEST:Barley provides facile access to BAC sequences and their annotations, along with the barley–Ae. tauschii synteny viewer. These BAC sequences constitute a resource to improve the efficiency of marker development, map-based cloning, and comparative genomics in barley and related crops. Additional knowledge about regions of the barley genome that are gene-dense but low recombination is particularly relevant.

Comments

Published version. Plant Journal, Vol. 84, No. 1 (October 2015): 216–227. DOI. © 2015 The Authors. Used with permission.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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