Document Type
Article
Language
eng
Format of Original
6 p.
Publication Date
1-1998
Publisher
Lippincott Williams & Wilkins, Inc.
Source Publication
Nursing Research
Source ISSN
0029-6562
Original Item ID
Shelves: RT1 .N8 Memorial Periodicals
Abstract
Background: The infusion of amphotericin-B (AmB) often produces clinically distressing rigors and chills, which promptly abate with intravenous injection of meperidine, although its mechanism of action is unknown.
Objective: To examine the effects of meperidine on the transcription or secretion of Interleukin 1β (IL-1β) in human mononuclear leukocytes (MNL) exposed in vitro to the lipopolysaccharide (LPS) contained inEscherichia coli endotoxin or to AmB.
Methods: Blood was drawn from eight healthy adult volunteers. The blood was centrifuged, and the layer containing MNL was separated; incubated with various combinations of medium, meperidine, and AmB; then tested for IL-1 content to determine the effect of meperidine on MNL secretion of IL-1β. To determine the effect on MNL transcription of IL-1β, the RNA was extracted from cells and the IL-1β was measured using one of two different methods.
Results: Incubation of human MNL in the presence of LPS or AmB significantly increased transcription of IL-1β mRNA and secretion of IL-1β. Addition of meperidine to these cultures significantly reduced LPS-induced, but not AmB-induced, secretion of IL-1β in vitro. Meperidine did not alter IL-1β mRNA levels in MNL exposed to LPS or AmB.
Conclusions: These data suggest that meperidine decreases rigors and chills in part by decreasing MNL secretion of IL-1β through a posttranscriptional mechanism.
Recommended Citation
McCarthy, Donna O.; Murray, Susan; Galagan, Dawn; Gern, James E.; and Hutson, Paul R., "Meperidine Attenuates the Secretion but Not the Transcription of IL-1ß in Human Mononuclear Leukocytes" (1998). College of Nursing Faculty Research and Publications. 226.
https://epublications.marquette.edu/nursing_fac/226
Comments
Accepted version. Nursing Research, Vol. 47, No. 1 (January/February 1998): 19-24. Permalink. © 1998 Lippincott- Raven Publishers. Used with permission.