Date of Award

Spring 1974

Degree Type

Thesis - Restricted

Degree Name

Master of Science (MS)




The metanephric kidney of mouse develops from the inductive interaction of two tissue components, the ureteric bud and the metanephrogenic mesenchyme. Considerable work has been done on the morphological changes occurring in the mesenchyme during tubulogenesis, however only limited studies have been conducted on changes occurring in the morphology of specific cell organelles and the activity of their respective enzymes. Since it has been established that peroxisomes have a widespread distribution in mammalian cells, it is of interest to study variations in the number and enzymatic activity of this organelle in differentiating cells. This information is of value not only in terms of peroxisome development, but also with respect to the activity of the organelle during differentiation. The term peroxisome designates a class of single membrane-bounded organelles characterized by the association of catalase with one-or-more hydrogen-peroxide generating oxidases. The specific enzymes of the peroxisome provide a suitable system for both biochemical and cytochemical analysis of organelle development. The study of peroxisomes during tubulogenesis may contribute to an improved understanding of the cellular processes required for normal kidney development.