Date of Award

Summer 1955

Degree Type

Thesis - Restricted

Degree Name

Master of Science (MS)



First Advisor

Laskowski, Michael


Previous attempts to purify the deoxyribonuclease from calf thymus (DNase II) have met with two obstacles. The enzyme is present in the fresh thymus in extremely small amounts, and in addition large mechanical losses are inherent in the methods of ammonium sulfate and alcohol fractionations. The recent development of a pair of ion-exchange resins of substituted cellulose (1) which have no apparent deleterious effect upon this sensitive protein suggested the possibility of chromatographic purification. The purpose of this study is to attempt to purify DNase II by the application of ion-exchange chromatography.



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