Date of Award

Spring 2009

Document Type

Thesis - Restricted

Degree Name

Master of Science (MS)



First Advisor

Bahcall, James K.

Second Advisor

Olsen, F. K.

Third Advisor

Maki, James


The purpose of this in vitro study was to investigate the antimicrobial activity of calcium hydroxide (Ca(OH)2; Ultradent. West Jordan, UT), 1%. 2%. and 4% chlorhexidine gel (CHX; Ultradent, West Jordan, UT), and combinations of calcium hydroxide and 2%, 4%, and 8% chlorhexidine gel (Ultradent, West Jordan, UT) against Enterococcus faecalis (American Type Culture Collection 4082). Twenty-eight Petri dishes of BHI agar were inoculated with E. faecalis. Each Petri dish had six saturated paper disks placed. Five of the paper disks had a different test medicament, and the last paper disk was saturated with sterile distilled water to serve as a control. Each medicament was tested a total of twenty times. Fourteen Petri dishes were incubated aerobically at 37°C for 48 hours, and fourteen Petri dishes were incubated anaerobically at 37°C for 48 hours. Each medicament was tested ten times aerobically and ten times anaerobically. The largest zone of bacterial inhibition was measured from the edge of the paper disk in millimeters and recorded. Statistical analysis was performed using ANOVA and significance was determined using the Tukey test. Calcium hydroxide only inhibited E. faecalis in direct contact. CHX and CHX/Ca(OH)2 mixed, regardless of the concentration of CHX, inhibited significantly more E. faecalis than Ca(OH)2 alone (p<0.001). CHX, regardless of concentration, inhibited statistically more E. faecalis than the mixture of CHX/Ca(OH)2 (p<0.001).



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