Date of Award


Document Type

Thesis - Restricted

Degree Name

Master of Science (MS)



First Advisor

William J. Stekiel

Second Advisor

David W. Glenister

Third Advisor

James J. Smith


The advance of instrumentation in biological sciences has aided scientists remarkably. The oxygen electrode is a polarographic device for measuring the partial pressure of oxygen in various media including blood, gas, and saline. A membrane covered polarographic electrode such as the Beckman Oxygen Needle Microelectrode is a small accurate instrument with profound applications and severe limitations. The evaluation of the applications and limitations of this electrode is the subject of this dissertation. The main points will be to:

1) establish the riged requirements for oxygen partial pressure measurement specifically in the media of blood, gas, and saline,

2) establish the temperature and stirring dependence of PO2 measurement,

3) establish a reliable, quick calibration procedure and

4) verify the reliability of in vivo and in vitro measurements.

In addition, the Beckman needle electrode is used in a new, rapid, simple, polarographic method for blood oxygen content determination.



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