Myosin-ATPase Fibre Typing of Chemically Skinned Muscle Fibres

Document Type

Article

Language

eng

Format of Original

6 p.

Publication Date

9-1985

Publisher

Kluwer

Source Publication

Journal of Molecular Histology

Source ISSN

1567-2379

Abstract

The efficacy of myosin (M)-ATPase fibre typing to differentiate fibre types in chemically (EGTA) skinned muscle fibres was investigated. Cryosections or single fibres from isolated bundles of chemically skinned rat extensor digitorum longus (EDL) and soleus (SOL) muscles were stained for M-ATPase activity. The results indicate that two major fibre types (type I and II, Brooke & Kaiser, 1970) can be indentified, as well as subgrouping of the type II fibres into types IIa and IIb. Thus, chemically skinning muscle fibres appears to have no detrimental effects on subsequent M-ATPase fibre typing.

Comments

Journal of Molecular Histology, Vol. 17, No. 9 (September 1985): 1021-1026. DOI.

Thomas Eddinger was affiliated with the University of Wisconsin - Madison at the time of publication.

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