Document Type

Article

Language

eng

Format of Original

3 p.

Publication Date

1990

Publisher

Oxford University Press

Source Publication

Nucleic Acids Research

Source ISSN

0305-1048

Original Item ID

doi: 10.1093/nar/18.22.6637

Abstract

The only well-characterized study of gene expression in Tetrahymena thermophila (1) demonstrates that the temperature dependent expression of the Ser H3 gene is regulated at the level of mRNA stability. A run-on transcription assay was developed to determine if regulation of RNA stability was a major mechanism regulating gene expression in Tetrahymena or if transcriptional regulation dominates. The relative transcriptional activities of 14 Tetrahymena genes were determined in different physiological/developmental states (growing, starved and conjugating) in which many of the genes showed striking differences in RNA abundance. In every case except Ser H3, changes in transcription accompanied changes in RNA abundance. Thus differential transcription, not differential RNA degradation, is the major mechanism regulating RNA abundance in Tetrahymena.

Comments

Published version. Nucleic Acids Research, Vol. 18, No. 22 (1990): 6637-6639. DOI. © Oxford University Press 1990. Used with permission.

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