Document Type

Article

Language

eng

Format of Original

7 p.

Publication Date

1-1992

Publisher

Society for Neuroscience

Source Publication

Journal of Neuroscience

Source ISSN

0270-6474

Abstract

When expressed in the Xenopus oocyte, the minK protein induces a slowly activating voltage-dependent potassium current (Isk). We studied the modulation of this current by altering intracellular cAMP levels and found that the amplitude of Isk is dramatically increased by treatments that raise cAMP levels and decreased by agents that lower cAMP levels. Preinjection of a protein inhibitor of the cAMP-dependent protein kinase blocked the effects of increased cAMP levels. There were no changes in the voltage dependence or kinetics of Isk. Mutations that eliminate a potential phosphorylation site on the minK protein did not block the effects of activating the kinase. In addition, the membrane capacitance of the oocyte increased and decreased in parallel with Isk. Our results fit a mechanism in which channel proteins are selectively inserted into and removed from the plasma membrane in response to changes in kinase activity.

Comments

Published version. Journal of Neuroscience, Vol. 12, No. 1 (January 1992): 290-296. Permalink. © 1992 Society for Neuroscience. Used with permission.

Edward Blumenthal was affiliated with the Yale University School of Medicine at the time of publication.

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