Document Type
Article
Language
eng
Format of Original
15 p.
Publication Date
1-1985
Publisher
Oxford University Press
Source Publication
Nucleic Acids Research
Source ISSN
0305-1048
Original Item ID
doi: 10.1093/nar/13.1.73
Abstract
We have investigated the timing of DNA synthesis, methylation and degradation during macronuclear development in the ciliate, Tetrahymena thermophila. DNA synthesis was first detected in the anlagen early in macronuclear development, but the majority of DNA synthesis occurred later, after pair separation. Anlagen DNA was first detectably methylated at GATC sites 3–5 hours after its synthesis. Once initiated, de novo methylation was rapid and complete, occurring between 13.5 and 15 hours of conjugation. The level of methylation of GATC sites was constant throughout the remainder of conjugation, and was similar to that in mock-conjugated cells. Degradation of DNA in the old macronucleus and DNA synthesis in the anlagen began at about the same time. Upon pair separation, less than 20% of old macronuclear DNA remained. A small percentage of nucleotides prelabeled prior to conjugation were recycled in the developing analgen.
Recommended Citation
Harrison, Gail Singer and Karrer, Kathleen M., "DNA Synthesis, Methylation and Degradation During Conjugation in Tetrahymena thermophila" (1985). Biological Sciences Faculty Research and Publications. 237.
https://epublications.marquette.edu/bio_fac/237
Comments
Published version. Nucleic Acids Research, Vol. 13, No. 1 (January 1985): 73-87. DOI. © 1985 Oxford University Press. Used with permission.
Kathleen Karrer was affiliated with Brandeis University at the time of publication.