Document Type
Article
Language
eng
Format of Original
11 p.
Publication Date
5-2011
Publisher
American Society for Microbiology
Source Publication
Journal of Bacteriology
Source ISSN
0021-9193
Original Item ID
doi: 10.1128/JB.01260-10; PubMed Central, PMCID: PMC3133073
Abstract
BacA of Sinorhizobium meliloti plays an essential role in the establishment of nitrogen-fixing symbioses with Medicago plants, where it is involved in peptide import and in the addition of very-long-chain fatty acids (VLCFA) to lipid A of lipopolysaccharide (LPS). We investigated the role of BacA in Rhizobium species strain NGR234 by mutating the bacA gene. In the NGR234 bacA mutant, peptide import was impaired, but no effect on VLCFA addition was observed. More importantly, the symbiotic ability of the mutant was comparable to that of the wild type for a variety of legume species. Concurrently, an acpXL mutant of NGR234 was created and assayed. In rhizobia, AcpXL is a dedicated acyl carrier protein necessary for the addition of VLCFA to lipid A. LPS extracted from the NGR234 mutant lacked VLCFA, and this mutant was severely impaired in the ability to form functional nodules with the majority of legumes tested. Our work demonstrates the importance of VLCFA in the NGR234-legume symbiosis and also shows that the necessity of BacA for bacteroid differentiation is restricted to specific legume-Rhizobium interactions.
Recommended Citation
Ardissone, Silvia; Kobayashi, Hajime; Kambara, Kumiko; Rummel, Coralie; Noel, K. Dale; Walker, Graham C.; Broughton, William J.; and Deakin, William J., "Role of BacA in Lipopolysaccharide Synthesis, Peptide Transport, and Nodulation by Rhizobium sp. Strain NGR234" (2011). Biological Sciences Faculty Research and Publications. 333.
https://epublications.marquette.edu/bio_fac/333
Comments
Published version. Journal of Bacteriology, Vol. 193, No. 9 (May 2011): 2218-2228. DOI. © 2011 American Society for Microbiology. Used with permission.