Document Type

Article

Language

eng

Format of Original

11 p.

Publication Date

5-2011

Publisher

American Society for Microbiology

Source Publication

Journal of Bacteriology

Source ISSN

0021-9193

Original Item ID

doi: 10.1128/JB.01260-10; PubMed Central, PMCID: PMC3133073

Abstract

BacA of Sinorhizobium meliloti plays an essential role in the establishment of nitrogen-fixing symbioses with Medicago plants, where it is involved in peptide import and in the addition of very-long-chain fatty acids (VLCFA) to lipid A of lipopolysaccharide (LPS). We investigated the role of BacA in Rhizobium species strain NGR234 by mutating the bacA gene. In the NGR234 bacA mutant, peptide import was impaired, but no effect on VLCFA addition was observed. More importantly, the symbiotic ability of the mutant was comparable to that of the wild type for a variety of legume species. Concurrently, an acpXL mutant of NGR234 was created and assayed. In rhizobia, AcpXL is a dedicated acyl carrier protein necessary for the addition of VLCFA to lipid A. LPS extracted from the NGR234 mutant lacked VLCFA, and this mutant was severely impaired in the ability to form functional nodules with the majority of legumes tested. Our work demonstrates the importance of VLCFA in the NGR234-legume symbiosis and also shows that the necessity of BacA for bacteroid differentiation is restricted to specific legume-Rhizobium interactions.

Comments

Published version. Journal of Bacteriology, Vol. 193, No. 9 (May 2011): 2218-2228. DOI. © American Society for Microbiology 2011. Used with permission.

Included in

Biology Commons

Share

COinS