Hemolytic Plaque Inhibition by Synthetic Antigenic Peptides of Sperm Whale Myoglobin

Document Type

Article

Language

eng

Format of Original

11 p.

Publication Date

1985

Publisher

Informa Healthcare

Source Publication

Immunological Investigations

Source ISSN

0882-0139

Abstract

We have investigated the systemic antibody response to sperm whale myoglobin (SWMb) antigenic sites in three strains of inbred mice using an inhibition of plaque assay. Sperm whale myoglobin was attached to sheep red blood cells (SRBC) via rabbit anti-SRBC Fab' fragments. Inhibition of lysis was obtained with synthetic peptides representing the purported five antigenic sites but not with a peptide whose sequence was unrelated to SWMb and synthetic peptides of SWMb from outside the antigenic sites gave minimal or no inhibition. The results of our studies show that the pattern of response to the five antigenic sites differs in each strain, but that almost total inhibition is obtained in all strains with these five sites. The antigenic dominance of these sites supports the concept of discrete antigenic sites on soluble proteins. They also suggest a reason for contrary reports in the literature based on hybridoma technology.

Comments

Immunological Investigations, Vol. 14, No. 4 (1985): 329-339. DOI.

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