L-Arabinose Isomerase Formation in a Conditional Mutant of Gene araA of Escherichia coli B/r

Document Type

Article

Language

eng

Format of Original

7 p.

Publication Date

12-1972

Publisher

American Society for Microbiology

Source Publication

Journal of Bacteriology

Source ISSN

0021-9193

Original Item ID

DOI:10.1128/jb.112.3.1247-1253.1972

Abstract

A temperature-sensitive mutant of Escherichia coli in which the synthesis of l-arabinose isomerase is blocked during growth at 42 C was found to possess the following properties. (i) The mutation occurred in the structural gene for the isomerase, gene araA. (ii) During growth at elevated temperatures the mutant accumulates a product which is a precursor to the active enzyme. (iii) The precursor produced at 42 C is slowly converted to active enzyme at 28 C in the absence of protein and ribonucleic acid synthesis. It is concluded that the mutation results in a change in the structure of isomerase which causes formation of active enzyme to be thermolabile at a step beyond the level of translation.

Comments

Published version. Journal of Bacteriology, Vol. 112, No. 3 (December 1972): 1247-1253. DOI. © 1972 American Society for Microbiology. Used with permission.

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