Complement Dependence of Human Serum Toxicity for Melanoma S91 Cells

Document Type




Format of Original

6 p.

Publication Date



National Academy of Sciences

Source Publication

Proceedings of the Society for Experimental Biology and Medicine

Source ISSN



Evidence is presented indicating that viable cells, freshly prepared from a solid mouse melanoma, are killed when reacted with normal human serum. Serum cytotoxicity was shown to be complement dependent by 1) heat lability, 2) residual complement testing, and 3) through diminution by reacting the serum with a secondary antigen for which it has a demonstrable antibody. Apparent absorption of toxicity from VDRL-reactive human sera by VDRL antigen was shown to be due to concomitant depletion of complement. The toxicity factor could not be correlated with “Wassermann” antibody or the sheep red blood cell antibody content of the sera. Furthermore, the cytotoxic activity could not be related to recent antibiotic therapy or clinically manifest venereal disease of the serum donors.


Proceedings of the Society for Experimental Biology and Medicine, Vol. 119, No. 4 (August 1965): 1112-1117. DOI.