Document Type
Article
Language
eng
Format of Original
11 p.
Publication Date
2013
Publisher
Society for the Study of Reproduction
Source Publication
Biology of Reproduction
Source ISSN
0006-3363
Original Item ID
DOI: 10.1095/biolreprod.112.106120
Abstract
Oocyte activation is an important process triggered by fertilization that initiates embryonic development. However, parthenogenetic activation can occur either spontaneously or with chemical treatments. The LT/Sv mouse strain is genetically predisposed to spontaneous activation. LT oocytes have a cell cycle defect and are ovulated at the metaphase I stage instead of metaphase II. A thorough understanding of the female meiosis defects in this strain remains elusive. We have reported that AMP-activated protein kinase (PRKA) has an important role in stimulating meiotic resumption and promoting completion of meiosis I while suppressing premature parthenogenetic activation. Here we show that early activation of PRKA during the oocyte maturation period blocked chemically induced activation in B6SJL oocytes and spontaneous activation in LT/SvEiJ oocytes. This inhibitory effect was associated with high levels of MAPK1/3 activity. Furthermore, stimulation of PRKA partially rescued the meiotic defects of LT/Sv mouse oocytes in concert with correction of abnormal spindle pole localization of PRKA and loss of prolonged spindle assembly checkpoint activity. Altogether, these results confirm a role for PRKA in helping sustain the MII arrest in mature oocytes and suggest that dysfunctional PRKA contributes to meiotic defects in LT/SvEiJ oocytes.
Recommended Citation
Ya, Ru and Downs, Stephen, "Suppression of Chemically Induced and Spontaneous Mouse Oocyte Activation by AMP-Activated Protein Kinase" (2013). Biological Sciences Faculty Research and Publications. 442.
https://epublications.marquette.edu/bio_fac/442
Comments
Accepted version. Biology of Reproduction, Vol. 88, No. 3(70) (2013): 1-11. DOI. © 2013 Society for the Study of Reproduction. Used with permission.