A Comparison of Sarcoplasmic Reticulum Function in Fast and Slow Skeletal Muscle Using Crude Homogenate and Isolated Vesicles

Authors

D. H. Kim, Marquette UniversityFollow
Frank Arthur Witzmann, Marquette UniversityFollow
Robert H. Fitts, Marquette UniversityFollow

Document Type

Article

Language

eng

Format of Original

7 p.

Publication Date

5-18-1981

Publisher

Elsevier

Source Publication

Life Sciences

Source ISSN

0024-3205

Abstract

Sarcoplasmic reticulum vesicles (FSR) were isolated from relatively homogeneous muscle samples representative of type I, IIA, and IIB fibers and Ca²⁺ uptake (V_max and total capacity) determined. Crude homogenates of these same fiber populations were also assayed for Ca²⁺ uptake and the results compared to the FSR values. Both techniques produced qualitatively similar results and demonstrated distinct fiber type differences in both the rate and extent of Ca²⁺ uptake. The results obtained support the contention that the crude homogenate technique accurately reflects the activity of the sarcoplasmic reticulum.

Comments

Life Sciences, Vol. 28, No. 20 (May 1981): 2223-2229. DOI.

Recommended Citation

Kim, D. H.; Witzmann, Frank Arthur; and Fitts, Robert H., "A Comparison of Sarcoplasmic Reticulum Function in Fast and Slow Skeletal Muscle Using Crude Homogenate and Isolated Vesicles" (1981). Biological Sciences Faculty Research and Publications. 487.
https://epublications.marquette.edu/bio_fac/487