Arabidopsis AZI1 Family Proteins Mediate Signal Mobilization for Systemic Defence Priming

Authors

Nicolas M. Cecchini, The University of Chicago
Kevin Steffes, The University of Chicago
Michael Schläppi, Marquette UniversityFollow
Andrew N. Gifford, Brookhaven National Laboratory
Jean T. Greenberg, The University of Chicago

Document Type

Article

Language

eng

Format of Original

12 p.

Publication Date

7-2015

Publisher

Nature Publishing Group

Source Publication

Nature Communications

Source ISSN

2041-1723

Abstract

Priming is a major mechanism behind the immunological 'memory' observed during two key plant systemic defences: systemic acquired resistance (SAR) and induced systemic resistance (ISR). Lipid-derived azelaic acid (AZA) is a mobile priming signal. Here, we show that the lipid transfer protein (LTP)-like AZI1 and its closest paralog EARLI1 are necessary for SAR, ISR and the systemic movement and uptake of AZA in Arabidopsis. Imaging and fractionation studies indicate that AZI1 and EARLI1 localize to expected places for lipid exchange/movement to occur. These are the ER/plasmodesmata, chloroplast outer envelopes and membrane contact sites between them. Furthermore, these LTP-like proteins form complexes and act at the site of SAR establishment. The plastid targeting of AZI1 and AZI1 paralogs occurs through a mechanism that may enable/facilitate their roles in signal mobilization.

Comments

Published version. nature Communications, Vol. 6, Issue 7658 (July 23, 2015): 1-12. DOI. © 2015 Macmillan Publishers Limited. Used with permission.

Recommended Citation

Cecchini, Nicolas M.; Steffes, Kevin; Schläppi, Michael; Gifford, Andrew N.; and Greenberg, Jean T., "Arabidopsis AZI1 Family Proteins Mediate Signal Mobilization for Systemic Defence Priming" (2015). Biological Sciences Faculty Research and Publications. 517.
https://epublications.marquette.edu/bio_fac/517