Electrochemical Approaches to the Study of Small Molecule-Protein Reaction Rates

Authors

Sipra Ray Dasgupta, Marquette University
Michael D. Ryan, Marquette UniversityFollow

Document Type

Article

Language

eng

Format of Original

8 p.

Publication Date

9-1980

Publisher

Elsevier

Source Publication

Bioelectrochemistry and Bioenergetics

Source ISSN

0302-4598

Abstract

The reduction kinetics of small molecule-protein reactions can be studied quite efficiently by the use of pulse polarography and differential pulse polarography. These results were obtained quite easily for relatively low concentrations of the mediator. It is only for differential pulse polarography that there is an absolute increase in the sensitivity when compared to chronoamperometry. This approach has been recently applied in our laboratory to low potential proteins such as spinach ferredoxin, where the requirements of low concentrations, small volumes and oxygen exclusion are quite critical. In addition, previous workers have shown that pulse and differential pulse polarography can be applied to stationary electrodes, which would greatly expand these approaches for small molecule-protein reaction studies.

Comments

Bioelectrochemistry and Bioenergetics, Vol. 7, No. 3 (September 1980): 587-594. DOI.

Recommended Citation

Dasgupta, Sipra Ray and Ryan, Michael D., "Electrochemical Approaches to the Study of Small Molecule-Protein Reaction Rates" (1980). Chemistry Faculty Research and Publications. 493.
https://epublications.marquette.edu/chem_fac/493