A Novel Electrochemical Biosensor Based On Fe3O4 Nanoparticles-Polyvinyl Alcohol Composite for Sensitive Detection of Glucose

Authors

Niuosha Sanaeifar, Amirkabir University of Technology
Mohammad Rabiee, Amirkabir University of Technology
Mojgan Abdolrahim, Amirkabir University of Technology
Mohammadreza Tahriri, Amirkabir University of TechnologyFollow
Daryoosh Vashaee, North Carolina State UniversityFollow
Lobat Tayebi, Marquette UniversityFollow

Document Type

Article

Language

eng

Format of Original

8 p.

Publication Date

2-15-2017

Publisher

Elsevier

Source Publication

Analytical Biochemistry

Source ISSN

0003-2697

Abstract

In this research, a new electrochemical biosensor was constructed for the glucose detection. Iron oxide nanoparticles (Fe₃O₄) were synthesized through co-precipitation method. Polyvinyl alcohol-Fe₃O₄ nanocomposite was prepared by dispersing synthesized nanoparticles in the polyvinyl alcohol (PVA) solution. Glucose oxidase (GOx) was immobilized on the PVA-Fe₃O₄ nanocomposite via physical adsorption. The mixture of PVA, Fe₃O₄ nanoparticles and GOx was drop cast on a tin (Sn) electrode surface (GOx/PVA-Fe₃O₄/Sn). The Fe₃O₄ nanoparticles were characterized by X-ray diffraction (XRD). Also, Fourier transform infrared (FTIR) spectroscopy and field emission scanning electron microscopy (FE-SEM) techniques were utilized to evaluate the PVA-Fe₃O₄ and GOx/PVA-Fe₃O₄ nanocomposites. The electrochemical performance of the modified biosensor was investigated using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Presence of Fe₃O₄nanoparticles in the PVA matrix enhanced the electron transfer between enzyme and electrode surface and the immobilized GOx showed excellent catalytic characteristic toward glucose. The GOx/PVA-Fe₃O₄/Sn bioelectrode could measure glucose in the range from 5 × 10⁻³ to 30 mM with a sensitivity of 9.36 μA mM⁻¹ and exhibited a lower detection limit of 8 μM at a signal-to-noise ratio of 3. The value of Michaelis-Menten constant (K_M) was calculated as 1.42 mM. The modified biosensor also has good anti-interfering ability during the glucose detection, fast response (10 s), good reproducibility and satisfactory stability. Finally, the results demonstrated that the GOx/PVA-Fe₃O₄/Sn bioelectrode is promising in biosensor construction.

Comments

Accepted version. Analytical Biochemistry, Vol. 519 (February 15, 2017): 19-26. DOI. © 2016 Elsevier Inc. Used with permission.

Recommended Citation

Sanaeifar, Niuosha; Rabiee, Mohammad; Abdolrahim, Mojgan; Tahriri, Mohammadreza; Vashaee, Daryoosh; and Tayebi, Lobat, "A Novel Electrochemical Biosensor Based On Fe3O4 Nanoparticles-Polyvinyl Alcohol Composite for Sensitive Detection of Glucose" (2017). School of Dentistry Faculty Research and Publications. 210.
https://epublications.marquette.edu/dentistry_fac/210