Nucleosome Positioning Is Independent of Histone H1 in Vivo
Format of Original
American Society for Biochemistry and Molecular Biology
Journal of Biological Chemistry
Nucleosome positioning in the somatic macronuclear genome of the ciliated protozoan Tetrahymena thermophila was analyzed by indirect end labeling. Nucleosomes were positioned nonrandomly in three different regions of theTetrahymena genome. Nucleosome repeat length varied between adjacent nucleosomes. Nucleosome positioning in a histone H1 knockout strain was indistinguishable from that in a strain with wild type histone H1.