Site-Specific Methylation of Adenine in the Nuclear Genome of a Eucaryote, Tetrahymena thermophila
Format of Original
American Society for Microbiology
Molecular and Cellular Biology
Original Item ID
DNA in the polyploid macronucleus of the ciliated protozoan Tetrahymena thermophila contains the modified base N6-methyladenine. We identified two GATC sites which are methylated in most or all of the 45 copies of the macronuclear genome. One site is 2 kilobases 5' to the histone H4-I gene, and the other is 5 kilobases 3' to the 73-kilodalton heat shock protein gene. These sites are de novo methylated between 10 and 16 h after initiation of conjugation, during macronuclear anlage development. The methylation states of these two GATC sites and four other unmethylated GATC sites do not change in the DNA of cells cultured under conditions which change the activity of the genes, including logarithmic growth, starvation, and heat shock.
Harrison, Gail Singer; Findly, R. Craig; and Karrer, Kathleen M., "Site-Specific Methylation of Adenine in the Nuclear Genome of a Eucaryote, Tetrahymena thermophila" (1986). Biological Sciences Faculty Research and Publications. 231.
Published version. Molecular and Cellular Biology, Vol. 6, No. 7 (July 1986): 2364-2370. DOI. © 1986 American Society for Microbiology. Used with permission.
Kathleen Karrer was affiliated with Brandeis University at the time of publication.