Title
A Comparison of Sarcoplasmic Reticulum Function in Fast and Slow Skeletal Muscle Using Crude Homogenate and Isolated Vesicles
Document Type
Article
Language
eng
Format of Original
7 p.
Publication Date
5-18-1981
Publisher
Elsevier
Source Publication
Life Sciences
Source ISSN
0024-3205
Abstract
Sarcoplasmic reticulum vesicles (FSR) were isolated from relatively homogeneous muscle samples representative of type I, IIA, and IIB fibers and Ca2+ uptake (Vmax and total capacity) determined. Crude homogenates of these same fiber populations were also assayed for Ca2+ uptake and the results compared to the FSR values. Both techniques produced qualitatively similar results and demonstrated distinct fiber type differences in both the rate and extent of Ca2+ uptake. The results obtained support the contention that the crude homogenate technique accurately reflects the activity of the sarcoplasmic reticulum.
Recommended Citation
Kim, D. H.; Witzmann, Frank Arthur; and Fitts, Robert H., "A Comparison of Sarcoplasmic Reticulum Function in Fast and Slow Skeletal Muscle Using Crude Homogenate and Isolated Vesicles" (1981). Biological Sciences Faculty Research and Publications. 487.
https://epublications.marquette.edu/bio_fac/487
Comments
Life Sciences, Vol. 28, No. 20 (May 1981): 2223-2229. DOI.