Cosmid Cloning of Five Zymomonas trp genes by Complementation of Escherichia coli and Pseudomonas putida trp Mutants

Christina Kay Eddy, Marquette University
Oliver H. Smith, Marquette University
K. Dale Noel, Marquette University

Published version. Journal of Bacteriology, Vol. 170, No. 7 (July 1988): 3158-3163. DOI. © 1988 American Society for Microbiology. Used with permission.


A library of Zymomonas mobilis genomic DNA was constructed in the broad-host-range cosmid pLAFR1. The library was mobilized into a variety of Escherichia coli and Pseudomonas putida trp mutants by using the helper plasmid pRK2013. Five Z. mobilis trp genes were identified by the ability to complement the trp mutants. The trpF, trpB, and trpA genes were on one cosmid, while the trpD and trpC genes were on two separate cosmids. The organization of the Z. mobilis trp genes seems to be similar to the organization found in Rhizobium spp., Acinetobacter calcoaceticus, and Pseudomonas acidovorans. The trpF, trpB, and trpA genes appeared to be linked, but they were not closely associated with trpD or trpC genes.