Mapping of Complementation Groups Within a Rhizobium leguminosarum CFN42 Chromosomal Region Required for Lipopolysaccharide Synthesis

Authors

Joseph Richard Cava, Marquette UniversityFollow
Hong Tao, Marquette University
K. Dale Noel, Marquette UniversityFollow

Document Type

Article

Language

eng

Publication Date

3-1990

Publisher

Springer

Source Publication

Molecular and General Genetics MGG

Source ISSN

0026-8925

Abstract

A major genetic region specifying portions of the carbohydrate structure of Rhizobium leguminosarum CFN42 lipopolysaccharide was analyzed by Tn5 mutagenesis, constructing deletions in cloned DNA, restriction mapping, and complementation analysis. Mutations affecting lipopolysaccharide synthesis were arranged in nine complementation groups spanning 18 kb of DNA. One mutation resulted in O-polysaccharide-containing lipopolysaccharide having a slightly increased mobility in gel electrophoresis. This mutation did not affect the symbiosis with bean plants. The other mutations eliminated the 0-polysaccharide-containing lipopolysaccharide and resulted in strains defective in eliciting bean nodule development.

Comments

Molecular and General Genetics MGG, Vol. 221, No. 1 (March 1990): 125-128. DOI.

Recommended Citation

Cava, Joseph Richard; Tao, Hong; and Noel, K. Dale, "Mapping of Complementation Groups Within a Rhizobium leguminosarum CFN42 Chromosomal Region Required for Lipopolysaccharide Synthesis" (1990). Biological Sciences Faculty Research and Publications. 585.
https://epublications.marquette.edu/bio_fac/585