Adenosine Type 1 (A ) Receptors Mediate Protection Against Myocardial 1 Infarction Produced by Chronic, Intermittent Ingestion of Ethanol in Dogs

Authors

Franz Kehl, Medical College of Wisconsin
John G. Krolikowski, Medical College of WisconsinFollow
John F. LaDisa, Marquette UniversityFollow
Judy R. Kersten, Medical College of Wisconsin
David C. Warltier, Medical College of WisconsinFollow
Paul S. Pagel, Medical College of WisconsinFollow

Document Type

Article

Language

eng

Format of Original

8 p.

Publication Date

4-2003

Publisher

Elsevier

Source Publication

International Journal of Cardiology

Source ISSN

0167-5273

Original Item ID

doi: 10.1016/S0167-5273(02)00329-7

Abstract

Background: Chronic consumption of small amounts of ethanol protects myocardium from ischemic injury. We tested the hypothesis that adenosine type 1 (A₁) receptors mediate these beneficial effects.

Methods: Dogs (n=37) were fed with ethanol (1.5 g/kg) or water mixed with dry food twice per day for 12 weeks, fasted overnight before experimentation, and instrumented for measurement of hemodynamics. Dogs received intravenous drug vehicle (50% polyethylene glycol in 0.1 N sodium hydroxide and 0.9% saline over 15 min) or the selective A₁ receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX, 0.8 mg/kg over 15 min) and were subjected to a 60 min coronary artery occlusion followed by 3 h of reperfusion. Myocardial infarct size and transmural coronary collateral blood flow were measured with triphenyltetrazolium chloride staining and radioactive microspheres, respectively.

Results: The area at risk (AAR) for infarction was similar between groups. Pretreatment with ethanol significantly reduced infarct size to 13±2% (n=7) of the AAR as compared to control experiments (26±2%; n=7). DPCPX abolished the protective effects of ethanol pretreatment (30±3%; n=7) but had no effect in dogs that did not receive ethanol (25±2%; n=7). No differences in transmural coronary collateral blood flow were observed between groups.

Conclusions: The present findings indicate that chronic ingestion of small amounts of ethanol produces myocardial protection that persists after the discontinuation of ethanol. The results indicate that A₁ receptors mediate ethanol-induced preconditioning in dogs independent of alterations in systemic hemodynamics or coronary collateral blood flow.

Comments

Accepted version. International Journal of Cardiology, Vol. 88, No. 2-3 (April 2003): 175-182. DOI. © 2003 Elsevier. Used with permission.

John LaDisa was affiliated with the Medical College of Wisconsin at the time of publication.

Recommended Citation

Kehl, Franz; Krolikowski, John G.; LaDisa, John F.; Kersten, Judy R.; Warltier, David C.; and Pagel, Paul S., "Adenosine Type 1 (A ) Receptors Mediate Protection Against Myocardial 1 Infarction Produced by Chronic, Intermittent Ingestion of Ethanol in Dogs" (2003). Biomedical Engineering Faculty Research and Publications. 223.
https://epublications.marquette.edu/bioengin_fac/223