Document Type
Article
Publication Date
4-2013
Publisher
Public Library of Science (PLoS)
Source Publication
PLoS One
Source ISSN
1932-6203
Abstract
A wavelength selection method that combines an inverse Monte Carlo model of reflectance and a genetic algorithm for global optimization was developed for the application of spectral imaging of breast tumor margins. The selection of wavelengths impacts system design in cost, size, and accuracy of tissue quantitation. The minimum number of wavelengths required for the accurate quantitation of tissue optical properties is 8, with diminishing gains for additional wavelengths. The resulting wavelength choices for the specific probe geometry used for the breast tumor margin spectral imaging application were tested in an independent pathology-confirmed ex vivo breast tissue data set and in tissue-mimicking phantoms. In breast tissue, the optical endpoints (hemoglobin, -carotene, and scattering) that provide the contrast between normal and malignant tissue specimens are extracted with the optimized 8-wavelength set with <9% error compared to the full spectrum (450–600 nm). A multi-absorber liquid phantom study was also performed to show the improved extraction accuracy with optimization and without optimization. This technique for selecting wavelengths can be used for designing spectral imaging systems for other clinical applications.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Lo, Justin Y.; Brown, J. Quincy; Dhar, Sulochana; Yu, Bing; Palmer, Gregory M.; Jokerst, Nan M.; and Ramanujam, Nirmala, "Wavelength Optimization for Quantitative Spectral Imaging of Breast Tumor Margins" (2013). Biomedical Engineering Faculty Research and Publications. 633.
https://epublications.marquette.edu/bioengin_fac/633
ADA Accessible Version
Comments
Published version. PLoS One, Vol. 8, No. 4 (April 2013): e61767. DOI. © 2013 Lo et. al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.