Document Type
Article
Language
eng
Publication Date
1-31-2018
Publisher
Nature Publishing Group
Source Publication
Scientific Reports
Source ISSN
2045-2322
Abstract
The failure of axon regeneration in the CNS limits recovery from damage and disease. Members of the KLF family of transcription factors can exert both positive and negative effects on axon regeneration, but the underlying mechanisms are unclear. Here we show that forced expression of KLF6 promotes axon regeneration by corticospinal tract neurons in the injured spinal cord. RNA sequencing identified 454 genes whose expression changed upon forced KLF6 expression in vitro, including sub-networks that were highly enriched for functions relevant to axon extension including cytoskeleton remodeling, lipid synthesis, and bioenergetics. In addition, promoter analysis predicted a functional interaction between KLF6 and a second transcription factor, STAT3, and genome-wide footprinting using ATAC-Seq data confirmed frequent co-occupancy. Co-expression of the two factors yielded a synergistic elevation of neurite growth in vitro. These data clarify the transcriptional control of axon growth and point the way toward novel interventions to promote CNS regeneration.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Wang, Zimei; Mehra, Vatsal; Simpson, Matthew T.; Maunze, Brian; Eastwood, Dan C.; Holan, Lyndsey; Blackmore, Murray G.; and Venkatesh, Ishwariya, "KLF6 and STAT3 Co-Occupy Regulatory DNA and Functionally Synergize to Promote Axon Growth in CNS Neurons" (2018). Biomedical Sciences Faculty Research and Publications. 186.
https://epublications.marquette.edu/biomedsci_fac/186
Comments
Accepted version. Scientific Reports, Vol. 8 (2018) Article number 12565. DOI. © 2018 The Author(s) .
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