Collection of Mouse Brain Slices for Bioluminescence Imaging of Circadian Clock Networks

Authors

Jennifer A. Evans, Marquette UniversityFollow
David K. Welsh, University of California - San Diego
Alec J. Davidson, Morehouse School of MedicineFollow

Document Type

Article

Publication Date

2021

Publisher

Humana Press

Source Publication

Methods in Molecular Biology

Source ISSN

1064-3745

Abstract

Circadian rhythms in cellular function can be monitored in real time with bioluminescence imaging. In this approach, bioluminescence is produced by an enzymatic reaction, which can be used to report dynamic changes in gene or protein expression in living cells. Bioluminescence imaging in circadian experiments typically uses an ex vivo slice preparation, with the most commonly studied structure being the master clock in the suprachiasmatic nucleus (SCN) of the anterior hypothalamus. Here we describe procedures for dissecting and collecting SCN slices for bioluminescence imaging experiments.

Comments

Methods in Molecular Biology, Vol. 2130 (2021): 287-294. DOI.

Recommended Citation

Evans, Jennifer A.; Welsh, David K.; and Davidson, Alec J., "Collection of Mouse Brain Slices for Bioluminescence Imaging of Circadian Clock Networks" (2021). Biomedical Sciences Faculty Research and Publications. 206.
https://epublications.marquette.edu/biomedsci_fac/206