Differentiation Between Rat Brain and Mouse Vas Deferens Delta Opioid Receptors

Authors

Linda K. Vaughn, Marquette UniversityFollow
William S. Wire, University of Arizona
Peg Davis, University of Arizona
Yasuyuki Shimohigashi
Richard J. Knapp, University of ArizonaFollow
Victor J. Hruby, University of ArizonaFollow
Thomas F. Burks, University of Arizona
Henry I. Yamamura, University of ArizonaFollow

Document Type

Article

Language

eng

Format of Original

3 p.

Publication Date

2-1990

Publisher

Elsevier

Source Publication

European Journal of Pharmacology

Source ISSN

0014-2999

Original Item ID

doi: 10.1016/0014-2999(90)90556-L

Abstract

Certain enkephalin analogues, including those which contain the conformationally restricted amino acid E-(2R,3S)-cyclopropylphenylalanine ((2R,3S)-▿^EPhe), have been shown to have high affinity for brain δ opioid receptors but are much less active in mouse vas deferens bioassays. To investigate whether there are differences between δ opioid receptors in brain and mouse was deferens, the ability of a selective δ opioid compound. [D-Pen²,pCl-Phe⁴,D-Pen⁵]enkephalin (pCl-DPDPE), and [D-Ala²,(2R,3S)-▿^EPhe⁴,Leu⁵]enkephalin methyl ester (CP-OMe), to inhibit [³H]pCl-DPDPE binding in both rat brain and mouse vas deferens were measured. pCl-DPDPE recognized brain and mouse vas deferences binding sites with equal affinity, however, CP-OMe showed 33 fold lower affinity in mouse vas deferens compared to brain. This suggests that mouse vas deferens δ opioid receptors may be distinct from brain δ opioid receptors.

Comments

European Journal of Pharmacology, Vol. 177, No. 1-2 (February 1990): 99-101. DOI.

Recommended Citation

Vaughn, Linda K.; Wire, William S.; Davis, Peg; Shimohigashi, Yasuyuki; Knapp, Richard J.; Hruby, Victor J.; Burks, Thomas F.; and Yamamura, Henry I., "Differentiation Between Rat Brain and Mouse Vas Deferens Delta Opioid Receptors" (1990). Biomedical Sciences Faculty Research and Publications. 71.
https://epublications.marquette.edu/biomedsci_fac/71