Document Type
Article
Publication Date
12-19-2006
Publisher
American Chemical Society
Source Publication
Langmuir
Source ISSN
0743-7463
Abstract
Construction of biomotors is an exciting area of scientific research that holds great promise for the development of new technologies with broad potential applications in areas such as the energy industry and medicine. Herein, we demonstrate the fabrication of prealigned microarrays of motile Escherichia coli bacterial cells on SiOx substrates. To prepare these arrays, holed surfaces with a gold layer on the bottom of the holes were utilized. The attachment of bacteria to the holes was achieved via nonspecific interactions using poly-l-lysine hydrobromide (PLL). Our data suggest that a single motile bacterial cell can be selectively attached to an individual hole on a surface and bacterial cell binding can be controlled by altering the pH, with the greatest occupancy occurring at pH 7.8. Cells attached to hole arrays remained motile for at least 4 h. These data indicate that holed surface structures provide a promising footprint for the attachment of motile bacterial cells to form high-density site-specific functional bacterial microarrays.
Recommended Citation
Rozhok, Sergey; Fan, Zhifang; Nyamjav, Dorjderem; Liu, Chang; Mirkin, Chad A.; and Holz, Richard C., "Attachment of Motile Bacterial Cells to Prealigned Holed Microarrays" (2006). Chemistry Faculty Research and Publications. 313.
https://epublications.marquette.edu/chem_fac/313
Comments
Accepted version. Langmuir, Vol. 22, No. 26 (December 19, 2006): 11251-11254. DOI. © 2006 American Chemical Society. Used with permission.
Richard C. Holz was affiliated with Loyola University Chicago at the time of publication.