Analysis of Ultrafast Transient Absorption Spectroscopy Data using Integrative Data Analysis Platform: 1. Data Processing, Fitting, and Model Selection

Authors

Evgueni Kovriguine, Marquette UniversityFollow

Document Type

Article

Language

English

Publication Date

7-2017

Publisher

Cold Spring Harbor Laboratory Press

Source Publication

bioRxiv beta

Abstract

This manuscript describes a workflow for analysis of transient absorption (TA) spectroscopy data using Integrative Data Analysis Platforms (IDAP) software package. Time-dependent spectral series are analyzed through evaluation of the isosbestic point and kinetics of excited state and ground-state bleach decays. Model fitting and selection based on Akaike's Information Criterion is discussed. As a practical example, we analyze excitation decays of a common protein label, Alexa Fluor 647.

Comments

Accepted version. bioRxiv beta (July 2017). DOI. © 2017 Cold Spring Harbor Laboratory Press. Used with permission.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.

Recommended Citation

Kovriguine, Evgueni, "Analysis of Ultrafast Transient Absorption Spectroscopy Data using Integrative Data Analysis Platform: 1. Data Processing, Fitting, and Model Selection" (2017). Chemistry Faculty Research and Publications. 576.
https://epublications.marquette.edu/chem_fac/576