Document Type

Article

Language

English

Publication Date

7-2017

Publisher

Cold Spring Harbor Laboratory Press

Source Publication

bioRxiv beta

Abstract

This manuscript describes a workflow for analysis of transient absorption (TA) spectroscopy data using Integrative Data Analysis Platforms (IDAP) software package. Time-dependent spectral series are analyzed through evaluation of the isosbestic point and kinetics of excited state and ground-state bleach decays. Model fitting and selection based on Akaike's Information Criterion is discussed. As a practical example, we analyze excitation decays of a common protein label, Alexa Fluor 647.

Comments

Accepted version. bioRxiv beta (July 2017). DOI.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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Chemistry Commons

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