Applicability of Quantitative PCR for Determination of Removal Efficacy of Enteric Viruses and Cryptosporidium by Water Treatment Processes
Document Type
Article
Language
eng
Format of Original
8 p.
Publication Date
2010
Publisher
IWA Publishing
Source Publication
Journal of Water and Health
Source ISSN
1477-8920
Original Item ID
doi: 10.2166/wh.2009.208
Abstract
This study aims to assess the applicability of quantitative PCR (qPCR) for removal studies of adenovirus, coxsackievirus, echovirus and Cryptosporidium by water treatment processes. Bench-scale coagulation jar tests were performed using the enteric viruses and Cryptosporidium. Standard methods (conventional cell-culture methods for the viruses and an immunofluorescence assay (IFA) for Cryptosporidium) were used to compare to qPCR. A significant correlation between microbial removals determined by qPCR and the standard detection methods and an approximate 1:1 correlation were observed for the challenge microorganisms. The results indicated that qPCR could be a satisfactory alternate for microbial removal studies using a relative quantification approach.
Recommended Citation
Ryu, Hodon; Mayer, Brooke; and Abbaszadegan, Morteza, "Applicability of Quantitative PCR for Determination of Removal Efficacy of Enteric Viruses and Cryptosporidium by Water Treatment Processes" (2010). Civil and Environmental Engineering Faculty Research and Publications. 37.
https://epublications.marquette.edu/civengin_fac/37
Comments
Journal of Water and Health, Vol. 8, No. 1 (2010): 101-108. DOI.
Brooke Mayer was affiliated with the Arizona State University at the Tempe Campus at the time of publication.