Document Type

Article

Language

eng

Format of Original

8 p.

Publication Date

3-2007

Publisher

Centers for Disease Control and Prevention

Source Publication

Emerging Infectious Diseases

Source ISSN

1080-6040

Original Item ID

doi: 10.3201/eid1303.060549

Abstract

Human noroviruses cause severe, self-limiting gastroenteritis that typically lasts 24–48 hours. Because of the lack of suitable tissue culture or animal models, the true nature of norovirus pathogenesis remains unknown. We show that noroviruses can infect and replicate in a 3-dimensional (3-D), organoid model of human small intestinal epithelium. Cells grown on porous collage-coated beads under fluid shear conditions in rotating wall vessel bioreactors differentiate into 3-D architectures resembling both the morphologic and physiologic function of in vivo tissues. Microscopy, PCR, and fluorescent in situ hybridization provided evidence of norovirus infection. Cytopathic effect and norovirus RNA were detected at each of the 5 cell passages for genogroup I and II viruses. Our results demonstrate that the highly differentiated 3-D cell culture model can support the natural growth of human noroviruses, whereas previous attempts that used differentiated monolayer cultures failed.

Comments

Published version. Emerging Infectious Diseases, Vol. 13, No. 3 (March 2007): 396-403. DOI.

This material is declared a work of the U.S. Government and is not subject to copyright protection in the United States. Approved for public release; distribution is unlimited.

Brooke K. Mayer was affiliated with Arizona State University at the time of publication.

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