Format of Original
American Society for Microbiology
Journal of Clinical Microbiology
Original Item ID
DOI: 10.1128/JCM.00271-16; PMCID: PMC4844743
As an alternative to automated extraction, fecal specimens were processed by investigational lysis/heating (i.e., manual) and by chromatography/centrifugation (i.e., column) methods. ProGastro SSC and Shiga toxin-producing Escherichia coli (i.e., STEC) indeterminate rates for 101 specimens were 1.0% to 3.0% for automated, 11.9% for manual, and 24.8% to 37.6% for column methods. Following freeze-thaw of 247 specimens, indeterminate rates were 1.6% to 2.4% for manual and 0.8 to 5.3% for column methods. Mean processing times for manual and column methods were 30.5 and 69.2 min, respectively. Concordance of investigational methods with automated extraction was ≥98.8%.
Munson, Erik; Napierala, Maureen; Munson, Kimber L.; Bilbo, Dorothy; and Schulte, Michael A., "Application of Alternative Nucleic Acid Extraction Protocols to ProGastro SSCS Assay for Detection of Bacterial Enteric Pathogens" (2016). Clinical Lab Sciences Faculty Research and Publications. 22.