Development of a DNA-Liposome Complex for Gene Delivery Applications

Authors

Morteza Rasoulianboroujeni, Marquette UniversityFollow
G. Kupgan, Oklahoma State University
F. Moghadam, Arizona State University
A. Boughdachi, Amirkabir University of Technology
Payam Khoshkenar, University of Massachusetts Medical SchoolFollow
J. J. Ambrose, Louisiana Tech University
N. Kiaie, Amirkabir University of Technology
Daryoosh Vashaee, North Carolina State University
J. D. Ramsey, Oklahoma State University
Lobat Tayebi, Marquette UniversityFollow

Document Type

Article

Language

eng

Publication Date

6-1-2017

Publisher

Elsevier

Source Publication

Materials Science and Engineering: C

Source ISSN

0928-4931)

Abstract

The association structures formed by cationic liposomes and DNA(Deoxyribonucleic acid)-liposome have been effectively utilized as gene carriers in transfection assays. In this research study, cationic liposomes were prepared using a modified lipid film hydration method consisting of a lyophilization step for gene delivery applications. The obtained results demonstrated that the mean particle size had no significant change while the polydispersity (PDI) increased after lyophilization. The mean particle size slightly reduced after lyophilization (520 ± 12 nm to 464 ± 25 nm) while the PDI increased after lyophilization (0.094 ± 0.017 to 0.220 ± 0.004). In addition. The mean particle size of vesicles increases when DNA is incorporated to the liposomes (673 ± 27 nm). According to the Scanning Electron Microscopy(SEM) and transmission electron microscopy (TEM) images, the spherical shape of liposomes confirmed their successful preservation and reconstitution from the powder. It was found that liposomal formulation has enhanced transfection considerably compared to the naked DNA as negative control. Finally, liposomal formulation in this research had a better function than Lipofectamine® 2000 as a commercialized product because the cellular activity (cellular protein) was higher in the prepared lipoplex than Lipofectamine® 2000.

Comments

Accepted version. Materials Science and Engineering: C, Vol. 75 (June 1, 2017): 191-197. DOI. © 2018 Elsevier B.V. Used with permission.

Recommended Citation

Rasoulianboroujeni, Morteza; Kupgan, G.; Moghadam, F.; Boughdachi, A.; Khoshkenar, Payam; Ambrose, J. J.; Kiaie, N.; Vashaee, Daryoosh; Ramsey, J. D.; and Tayebi, Lobat, "Development of a DNA-Liposome Complex for Gene Delivery Applications" (2017). School of Dentistry Faculty Research and Publications. 314.
https://epublications.marquette.edu/dentistry_fac/314