Document Type
Article
Language
eng
Publication Date
12-2019
Publisher
Springer-Verlag
Source Publication
Microchimica Acta
Source ISSN
0026-3672
Abstract
An electrochemical aptasensor is described for the voltammetric determination of lipopolysaccharide (LPS) from Escherichia coli 055:B5. Aptamer chains were immobilized on the surface of a glassy carbon electrode (GCE) via reduced graphene oxide and gold nanoparticles (RGO/AuNPs). Fast Fourier transform infrared, X-ray diffraction and transmission electron microscopy were used to characterize the nanomaterials. Cyclic voltammetry, square wave voltammetry and electrochemical impedance spectroscopy were used to characterize the modified GCE. The results show that the modified electrode has a good selectivity for LPS over other biomolecules. The hexacyanoferrate redox system, typically operated at around 0.3 V (vs. Ag/AgCl) is used as an electrochemical probe. The detection limit is 30 fg·mL−1. To decrease the electrochemical potential for detection of LPS, Mg/ carbon quantum dots were used as redox active media. They decrease the detection potential to 0 V and the detection of limit (LOD) to 1 fg·mL−1. The electrode was successfully used to analyze serum of patients and healthy persons.
Recommended Citation
Pourmadadi, Mehrab; Shayeh, Javad Shabani; Omidi, Meisam; Yazdian, Fatemeh; Alebouyeh, Masoud; and Tayebi, Lobat, "A Glassy Carbon Electrode Modified with Reduced Graphene Oxide and Gold Nanoparticles for Electrochemical Aptasensing Of Lipopolysaccharides from Escherichia Coli Bacteria" (2019). School of Dentistry Faculty Research and Publications. 362.
https://epublications.marquette.edu/dentistry_fac/362
Comments
Accepted version. Microchimica Acta, Vol. 186, No. 12 (December 2019). DOI. © 2019 Springer-Verlag GmbH Austria, part of Springer Nature. Used with permission.