Synthesis Of Novel Reducing Agent for Formation of Metronidazole-Capped Silver Nanoparticle and Evaluating Antibacterial Efficiency in Gram-Positive and Gram-Negative Bacteria

Authors

Fatemeh Farjadian, Shiraz University of Medical Sciences
Amin Reza Akbarizadeh, Shiraz University of Medical Sciences
Lobat Tayebi, Marquette UniversityFollow

Document Type

Article

Publication Date

8-2020

Publisher

Elsevier

Source Publication

Heliyon

Source ISSN

2405-8440

Abstract

In this study, a new type of silver nanoparticles capped with metronidazolium based ionic liquid is synthesized. By this aim, metronidazole is altered to ionic-liquid type structure with citrate counter ion as reducing agent. The produced reducing agent was characterized using ¹HNMR and ¹³CNMR and FT-IR. The capability of metronidazolium-based reducing agent in formation and capping silver nanoparticles was examined in a chemical reaction. More specifically, synthesized silver nanoparticles were synthesized and capped with metronidazolium-citrate based ionic liquid, while the formation of particles in 48 h was monitored by UV-Vis spectroscopy. Fourier transform infrared spectroscopy showed the presence of capping agents around silver nanoparticles. The amount of metronidazolium and citrate as capping agents was determined by thermal gravimetric analysis. The prepared crystalline structure of silver nanoparticles was proved by X-ray diffraction spectroscopy. PSA analysis and TEM was performed to determine the size of particles. The synthesized silver nanoparticle has the potential to be used as an antibacterial agent in preparation of wound dressing with extra capability and efficacy in aerobic and anaerobic bacterium. In this regard, the antibacterial efficacy of discs from different concentration of silver nanoparticles in calcium alginate medium were evaluated in Gram-negative and Gram-positive bacterium.

Comments

Published version. Heliyon, Vol. 6, No. 8 (August 2020): e04747. DOI. © 2020 Authors. Published by Elsevier Ltd. Used with permission.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.

Recommended Citation

Farjadian, Fatemeh; Akbarizadeh, Amin Reza; and Tayebi, Lobat, "Synthesis Of Novel Reducing Agent for Formation of Metronidazole-Capped Silver Nanoparticle and Evaluating Antibacterial Efficiency in Gram-Positive and Gram-Negative Bacteria" (2020). School of Dentistry Faculty Research and Publications. 421.
https://epublications.marquette.edu/dentistry_fac/421