Critical-Sized Bone Defects Regeneration Using a Bone-Inspired 3D Bilayer Collagen Membrane in Combination with Leukocyte and Platelet-Rich Fibrin Membrane (L-PRF): An In Vivo Study

Authors

Farahnaz Fahimipour, Marquette University
Farshid Bastami, Shahid Beheshti University
Ahad Khoshzaban, Tehran University of Medical Sciences
Shahrbanoo Jahangir, Iran University of Medical SciencesFollow
Mohamadreza Baghaban Eslaminejad, Royan Institute for Stem Cell Biology and Technology, TehranFollow
Fahimeh Kahyyatan, Royan Institute for Stem Cell Biology and Technology
Hannaneh Safiaghdam, Shahid Beheshti University of Medical Sciences
Yeganeh Sadooghi, Tehran University of Medical Sciences
Majid Safa, Iran University of Medical Sciences
Tahereh S. Jafarzadeh Kashi, Tehran University of Medical Sciences
Erfan Dashtimoghadam, Marquette UniversityFollow
Lobat Tayebi, Marquette UniversityFollow

Document Type

Article

Publication Date

4-2020

Publisher

Elsevier

Source Publication

Tissue and Cell

Source ISSN

0040-8166

Abstract

Objectives

We aim to develop a 3D-bilayer collagen (COL) membrane reinforced with nano beta-tricalcium-phosphate (nβ-TCP) particles and to evaluate its bone regeneration in combination with leukocyte-platelet-rich fibrin (L-PRF) in vivo.

Background data

L-PRF has exhibited promising results as a cell carrier in bone regeneration in a number of clinical studies, however there are some studies that did not confirm the positive results of L-PRF application.

Methods

Mechanical & physiochemical characteristics of the COL/nβ-TCP membrane (1/2 & 1/4) were tested. Proliferation and osteogenic differentiation of seeded cells on bilayer collagen/nβ-TCP thick membrane was examined. Then, critical-sized calvarial defects in 8 white New Zealand rabbits were filled with either Col, Col/nβ-TCP, Col/nβ-TCP combined with L-PRF membrane, or left empty. New bone formation (NBF) was measured histomorphometrically 4 & 8 weeks postoperatively.

Results

Compressive modulus increases while porosity decreases with higher β-TCP concentrations. Mechanical properties improve, with 89 % porosity (pore size ∼100 μm) in the bilayer-collagen/nβ-TCP membrane. The bilayer design also enhances the proliferation and ALP activity. In vivo study shows no significant difference among test groups at 4 weeks, but Col/nβ-TCP + L-PRF demonstrates more NBF compared to others (P < 0.05) after 8 weeks.

Conclusion

The bilayer-collagen/nβ-TCP thick membrane shows promising physiochemical in vitro results and significant NBF, as ¾ of the defect is filled with lamellar bone when combined with L-PRF membrane.

Comments

Accepted version. Tissue and Cell, Vol. 63 (April 2020): 101326. DOI. © 2020 Elsevier. Used with permission.

Recommended Citation

Fahimipour, Farahnaz; Bastami, Farshid; Khoshzaban, Ahad; Jahangir, Shahrbanoo; Eslaminejad, Mohamadreza Baghaban; Kahyyatan, Fahimeh; Safiaghdam, Hannaneh; Sadooghi, Yeganeh; Safa, Majid; Kashi, Tahereh S. Jafarzadeh; Dashtimoghadam, Erfan; and Tayebi, Lobat, "Critical-Sized Bone Defects Regeneration Using a Bone-Inspired 3D Bilayer Collagen Membrane in Combination with Leukocyte and Platelet-Rich Fibrin Membrane (L-PRF): An In Vivo Study" (2020). School of Dentistry Faculty Research and Publications. 444.
https://epublications.marquette.edu/dentistry_fac/444