Concurrent Application of Conductive Biopolymeric Chitosan/ Polyvinyl Alcohol/ MWCNTs Nanofibers, Intracellular Signaling Manipulating Molecules and Electrical Stimulation for more Effective Cardiac Tissue Engineering

Authors

Ali Abedi, University of Tehran
Behnaz Bakhshandeh, University of Tehran
Ali Babaie, University of Tehran
Javad Mohammadnejad, University of Tehran
Sadaf Vahdat, Tarbiat Modares University
Reza Mombeiny, Iran University of Medical Sciences
Seyed Reza Moosavi, Tehran University of Medical Sciences
Javid Amini, Islamic Azad University
Lobat Tayebi, Marquette UniversityFollow

Document Type

Article

Publication Date

1-2021

Publisher

Elsevier

Source Publication

Materials Chemistry and Physics

Source ISSN

0254-0584

Abstract

Fabrication of appropriate electro-conductive scaffold, application of small molecules (SMs), electrical stimulation (ES), and stem cells are steps forward in cardiac tissue engineering. Herein, for the first time, all mentioned factors have been taken into account concurrently regarding the differentiation of unrestricted somatic stem cells (USSCs) into cardiac cells. To accomplish this goal, electrospun composite scaffolds made of chitosan (CS) and polyvinyl alcohol (PVA) with multi-wall carbon nanotubes (MWCNTs; ranged from 0 to 2.5% w/w) were fabricated. After analyzing mechanical, electrical, and biological properties, the best MWCNTs portion was selected. Of note, the addition of 2%w/w MWCNTs to the CS/PVA samples reduced average fiber diameter from 225 to 110 nm, increasing electrical conductivity from 8 × 10⁻⁵ S/m to 9 × 10⁻³ S/m and trebling tensile strength. Then, by using a 10-day differentiation protocol (including CHIR99021, IWP2, SB431542, and purmorphamine SMs) and ES, USSCs were induced into cardiomyocytes. Overexpression of some cardiac-associated genes, including troponin I, CX43, and β-MHC, along with proper phenotypic alteration, were observed. (Scaffold + SM + ES) show a significant increase in the expression of these genes, 172, 5.3, and 64-times as normalized to undifferentiated cells, respectively. Our findings confirmed the importance of the simultaneous implementation of different factors for the developing functionality of the cardiac tissue. Altogether, it is recommended to deploy all mentioned features to obtain effective cardiac tissue engineering.

Comments

Accepted version. Materials Chemistry and Physics, Vol. 258 (January 2021): 123842. DOI. © 2021 Elsevier. Used with permission.

Recommended Citation

Abedi, Ali; Bakhshandeh, Behnaz; Babaie, Ali; Mohammadnejad, Javad; Vahdat, Sadaf; Mombeiny, Reza; Moosavi, Seyed Reza; Amini, Javid; and Tayebi, Lobat, "Concurrent Application of Conductive Biopolymeric Chitosan/ Polyvinyl Alcohol/ MWCNTs Nanofibers, Intracellular Signaling Manipulating Molecules and Electrical Stimulation for more Effective Cardiac Tissue Engineering" (2021). School of Dentistry Faculty Research and Publications. 503.
https://epublications.marquette.edu/dentistry_fac/503