Comparison of Osteogenic Differentiation Potential of Induced Pluripotent Stem Cells and Buccal Fat Pad Stem Cells on 3D-Printed HA/β-TCP Collagen-Coated Scaffolds

Authors

Sheida Hashemi, Shahid Beheshti University of Medical Sciences
Leila Mohammadi Amirabad, Marquette University
Saeed Farzad-Mohajeri, University of Tehran
Maryam Rezai Rad, Shahid Beheshti University of Medical Sciences
Farahnaz Fahimipour, Marquette University
Abdolreza Ardeshirylajimi, Shahid Beheshti University of Medical Sciences
Erfan Dashtimoghadam, Marquette UniversityFollow
Mohammad Salehi, Shahid Beheshti University of Medical Sciences
Masoud Soleimani, Tarbiat Modares UniversityFollow
Mohammad Mehdi Dehghan, University of Tehran
Lobat Tayebi, Marquette UniversityFollow
Arash Khojasteh, Shahid Beheshti University

Document Type

Article

Publication Date

5-2021

Publisher

Springer

Source Publication

Cell and Tissue Research

Source ISSN

0302-766x

Abstract

Production of a 3D bone construct with high-yield differentiated cells using an appropriate cell source provides a reliable strategy for different purposes such as therapeutic screening of the drugs. Although adult stem cells can be a good source, their application is limited due to invasive procedure of their isolation and low yield of differentiation. Patient-specific human-induced pluripotent stem cells (hiPSCs) can be an alternative due to their long-term self-renewal capacity and pluripotency after several passages, resolving the requirement of a large number of progenitor cells. In this study, a new biphasic 3D-printed collagen-coated HA/β-TCP scaffold was fabricated to provide a 3D environment for the cells. The fabricated scaffolds were characterized by the 3D laser scanning digital microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and mechanical test. Then, the osteogenesis potential of the hiPSC-seeded scaffolds was investigated compared to the buccal fat pad stem cell (BFPSC)-seeded scaffolds through in vitro and in vivo studies. In vitro results demonstrated up-regulated expressions of osteogenesis-related genes of RUNX2, ALP, BMP2, and COL1 compared to the BFPSC-seeded scaffolds. In vivo results on calvarial defects in the rats confirmed a higher bone formation in the hiPSC-seeded scaffolds compared to the BFPSC-seeded groups. The immunofluorescence assay also showed higher expression levels of collagen I and osteocalcin proteins in the hiPSC-seeded scaffolds. It can be concluded that using the hiPSC-seeded scaffolds can lead to a high yield of osteogenesis, and the hiPSCs can be used as a superior stem cell source compared to BFPSCs for bone-like construct bioengineering.

Comments

Cell and Tissue Research, Vol. 384, No. 2 (May 2021): 403-421. DOI.

Recommended Citation

Hashemi, Sheida; Amirabad, Leila Mohammadi; Farzad-Mohajeri, Saeed; Rad, Maryam Rezai; Fahimipour, Farahnaz; Ardeshirylajimi, Abdolreza; Dashtimoghadam, Erfan; Salehi, Mohammad; Soleimani, Masoud; Dehghan, Mohammad Mehdi; Tayebi, Lobat; and Khojasteh, Arash, "Comparison of Osteogenic Differentiation Potential of Induced Pluripotent Stem Cells and Buccal Fat Pad Stem Cells on 3D-Printed HA/β-TCP Collagen-Coated Scaffolds" (2021). School of Dentistry Faculty Research and Publications. 507.
https://epublications.marquette.edu/dentistry_fac/507